WebAug 1, 2012 · The goal of this project is to develop HTS assays that will allow discovery of small molecule inhibitors of DHX9, which in turn, can be used in cell and animal models to examine the consequences of inhibition of this protein on cancer cell survival. ... Pelletier, Jerry (2015) Establishment of a Primary Screening Assay for the DHX9 Helicase ... WebKeywords: ATPase assay, ATA, aurintricarboxylic acid, DHX9, HTS, RNA binding, RNA helicase. Abstract: The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from genome stability and replication, to transcriptional ...
DNA-sensing–independent role of a nuclear RNA helicase, DHX9, in sti…
Web13211 Ensembl ENSG00000135829 ENSMUSG00000042699 UniProt Q08211 O70133 RefSeq (mRNA) NM_001357 NM_030588 NM_007842 RefSeq (protein) NP_001348 NP_031868 NP_001392438 Location (UCSC) Chr 1: 182.84 – 182.89 Mb Chr 1: 153.33 – 153.36 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse ATP-dependent … WebJul 5, 2016 · Initial helicase assays demonstrated double-stranded DNA unwinding activity in bovine DHX9 and RNA unwinding activity with the human orthologue; however, … small green bugs on pepper plants
Targeting the DHX9 Helicase for Small Molecule Probe Discovery
WebJul 23, 2010 · The SAT motif has been shown by mutagenesis to uncouple ATPase activity from RNA helicase activity in both DEAD-box 46, 47, ... NTPase assay. DHX9 325–840 was diluted into an assay buffer [20 mM Hepes (pH 7.5), 3 mM MgCl 2, 300 mM NaCl, 10% glycerol, and 2 mM TCEP] up to final concentrations of 7–80 nM. The assay was … WebOct 22, 2024 · DHX9 shares common functions with CIZ1, including interaction with XIST long-non-coding RNA, epigenetic maintenance and regulation of DNA replication. ... Products of GST-CIZ1 interaction assay ... WebOct 17, 2006 · Interacts (via DRBM, OB-fold and RGG regions) with NUP98 (via N-terminus); this interaction occurs in a RNA-dependent manner and stimulates DHX9-mediated ATPase activity and regulates transcription and splicing of a subset of genes (PubMed:28221134). small green bugs that bite