Fastpfu polymerase
WebApr 12, 2024 · FastPfu DNA polymerase and 2× FastPfu reaction buffer (Tolo Biotech., China) ( see Note 3 ). 4. 10 mM dNTP working solution. 5. 0.2 mL thin-walled PCR tubes. 6. 1.5 mL polypropylene microcentrifuge tubes. 7. Thermocycler with heated lid ( see Note 4 ). 8. PCR Clean-Up System. 9. Distilled Water, DNase, RNase-free ( see Note 5 ). 10. WebMar 31, 2024 · The PCR (TransGen AP221-02: TransStart Fastpfu DNA Polymerase) program began with a denaturation step (94 °C; 3 min), followed by denaturation (20 cycles of 1 min; 94 °C), annealing (1 min; 65 °C to 57 °C with a 1°C reduction every two cycles—one cycle at 56 °C, and one cycle at 55 °C), elongation steps (72 °C; 1 min), and …
Fastpfu polymerase
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WebBrand : TransGen Biotech. Manual. Product Details. TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized … WebThe PCR mixtures contain 5 × TransStart FastPfu buffer 4 μL, 2.5 mM dNTPs 2 μL , forward primer (5 μM) 0.8 μL, reverse primer (5 μM) 0.8 μL, TransStart FastPfu DNA …
WebNov 13, 2024 · The V3-V4 regions of the 16S ribosomal RNA gene were amplified by PCR. The reaction mixture contained 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM … WebJul 22, 2024 · PCR amplification was performed using the TransStart ® FastPfu Polymerase system (Transgen Biotech, Beijing, China), including 4 μL 5 × FastPfu Buffer, 2 μL dNTPs (2.5 mM), 0.8 μL each primer (5 μM), 0.4 μL FastPfu Polymerase, and 10 ng template DNA. The reaction mixture was initially denatured at 95°C for 2 min, followed by …
WebThe PCR reactions were performed in triplicate 20 μL of mixtures containing 0.4 μL of FastPfu polymerase, 4 μL of 5 × FastPfu buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), and 10 ng of template DNA. The amplicons were purified from 2% (w/v) agarose gels using an AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City ... WebApr 11, 2024 · PCR reactions were performed in quadruplicate using a 20 μL mixture consisting of 4 μL of 5× TransStart FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of Forward Primer and Reverse Primer (5 μM), 0.4 μL of FastPfu Polymerase, 0.2 μL BSA, 10 ng Template DNA, and supplemented with sterile water to 20 μL. The qPCR product was …
WebDec 23, 2024 · Based on polymerase chain reaction (PCR) method and 16S rRNA sequence similarity values, ... 0.8 μL of forward and reverse primers (5 μM), 0.4 μL of FastPfu Polymerase and 10 ng of template DNA were for PCR amplification in triplicate. PCR amplicons were purified by AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, …
Web侯 磊，薛 蓓，薛会英 (西藏农牧学院，西藏 林芝 860000) 土壤线虫作为土壤动物中的优势类群，是草地生态系统中的消费者，关系到草地生产力的增减[1]。 j.crew toothpick jeansWebJul 15, 2024 · Planktonic eukaryotes are important components of aquatic ecosystems, and analyses of the whole eukaryotic planktonic community composition and function have far-reaching significance for water resource management. We aimed to understand the spatiotemporal variation and drivers of eukaryotic plankton distribution in the Middle … lsw music calendarWeb本发明公开一种基于环境DNA技术监测淡水底栖动物群落多样性的方法，包括步骤：(1)采集表层水样；(2)水样过滤与eDNA提取；(3)PCR扩增；(4)高通量测序，获得OTU的代表序列；(5)底栖动物比对数据库的建立；(6)比对建立的底栖动物数据库，对待测样品OTUs代表性序列进行物种注释。本发明取样简单、人力 ... lswo medicalWebTransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfu Fly DNA Polymerase. l s worldWebApr 10, 2024 · PCR was performed in triplicate in a 20 μL mixture containing 4 μL of 5 × FastPfu Buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of each primer (5 μM), 0.4 μL of FastPfu Polymerase, and 10 ng of template DNA. j crew toronto storesWebTransStart® FastPfu DNA Polymerase 是用于快速PCR的热启动高保真DNA聚合酶，TransStart® FastPfu DNA Polymerase扩增效率高、扩增速度快(4 kb/min，是普通Pfu … j crew torontoWebTransStart® FastPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. j crew top wool coat