Webb1 juli 2006 · The PCR product was ligated with pGEM-T Easy plasmid, resulting in plasmid pGM.HisRep. The 1,085-nt fragment was excised from the plasmid pGM.HisRep by using BglII and placed under control of the polyhedron promoter of the pFastBac Dual donor plasmid that had been linearized with BamHI, yielding plasmid pFBD.HisRep/O. http://kritzerland.com/KL_TLS_Notes.pdf
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