Phis2.1 vector
Webb一、载体概念. 1. 载体，DNA的搬运工. 载体：在基因工程重组DNA技术中，将DNA片段（目的基因）转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体，基因密码承载者. 载 … WebbLearn Biology Online. Biology Online is the world’s most comprehensive database of Biology terms and topics. Since 2001 it has been the resource of choice for professors, students, and professionals needing answers to Biology questions. Search over 75,000+ terms, news, insights, discoveries, and trends in Biology below:
Phis2.1 vector
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Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use … WebbBackground Drought is ampere major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors exist plant-specific proteins that play diverse roles in plant development. However, that functions of a count of GRAS genes identified in oats are unknown, specifically the GRASS genes related for fruit drought resistance have not …
WebbDescription pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ OneHybrid Library Construction & Screening Kit (#K1617-1). Webb13 juni 2015 · GTP1-GTP8 indicates the pGAD-OsGRAS23 plus pHIS2.1-cis (promoters of Os07g0162450, Os01g0537250, Os07g0638400 ... the promoter sequences were cloned into the yeast expression vector pHIS2.1 between the SmaI and EcoRI sites. The constructs were co-transformed into the yeast Y187 (MATa; ura3–52; his3–200; …
Webb16 mars 2024 · Y187-pHis2系统酵母单杂培养基套装说明书. 储存温度：序号1-7常温保存；序号8需-20℃储存，蓝冰运输. 产品组成：（0.5 L/pouch） 产品说明： Y187-pHis2 … http://www.biovector.net/product/319746.html
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WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. … dynamic grid reacthttp://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html in cell b10 add the total amountsWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … in cell b10 enter a formula using nperWebb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … dynamic business law 4th edition kubasekWebb25 okt. 2016 · In the mutated assay, the sequence of the G-box in the NWRE (GCGTTCTTACTTTTCGTAAGGG CACGTG AAGCACCCCAACAAA) was replaced with … dynamic dns credentialsWebb5 juli 2024 · The empty vector pHIS2.1 was used as the negative control. All cells carrying different vector combinations grew normally and equally on the SD/-Leu/-Trp medium (Fig. 4 b). However, the growth of yeast cells with the combination of pGADT7/pHIS2.1 empty vectors or pGADT7- GsERF71 /pHIS2.1 vectors was significantly inhibited on the SD/ … in cell b10 enter a formula using pvWebbCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. … in ceiling tweeter direction speakers